聚乙烯醇微透镜阵列的制备及其固载细胞色素c的直接电化学

Fabrication of Polyvinyl Alcohol Arrays and the Direct Electrochemistry of Adsorbed Cytochrome c

利用自组装法以聚苯乙烯有序多孔膜为膜板制备聚乙烯醇微透镜阵列膜.在pH6.89的磷酸盐(PBS)缓冲溶液中,固载在聚乙烯醇微透镜阵列膜修饰的玻碳电极上的细胞色素c于0.072V(vs·Ag/AgCl)处显示一对准可逆的氧化还原峰,是细胞色素c血红素辅基Fe(Ⅲ)/Fe(Ⅱ)电对的特征峰.考察了扫速、溶液pH及支持电解质浓度等因素对细胞色素c电子传递的影响,体系的表观异相电子传递速率常数k0为2.98×10-6cm·s-1.

Regular polyvinyl alcohol arrays were prepared with porous polystyrene films by a self-assembly method. Cyt c immobilized on PVA microlens arrays modified glassy carbon eleetrodes gave a pair of stable, well-defined and quasi-reversible cyclic vohammetrie peaks at about 0.072 V（ vs. Ag/AgCl） in 0.1mol/L PBS buffer （pH = 6.89） solution. The effects of scan rate, pH and concentration of buffers on the electrochemical behaviors of Cyt c were studied in detail. The apparent heterogeneous electron transfer rate constant （k^0） was evaluated to be 2.98×10^-6cm·s^-1.