妊娠期高血压疾病患者载脂蛋白C3基因单核苷酸多态性

Study on mononucleotide polymorphism of apolipoprotein C3 gene in patients with hypertensive disorder complicating pregnancy

目的:探讨载脂蛋白C3(apolipoprotein C3)第四外显子3’非编码区第3 175位(C→G)SstⅠ单核苷酸多态性(single nucleotide polymorphism,SNP)与妊娠期高血压疾病患者发病的关系。方法:采用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)技术,检测84例妊娠期高血压疾病患者(妊娠期高血压疾病组,其中妊娠期高血压28例、子痫前期-轻度13例、子痫前期-重度32例、子痫11例)和81例正常晚期妊娠妇女(正常孕妇组)的载脂蛋白C3第3 175位SstⅠ基因型。结果:①妊娠期高血压疾病组孕妇载脂蛋白C3基因第3 175位基因型频率CC、GC、GG分别为48.81%、36.90%、14.29%,正常孕妇组分别为54.32%、35.80%、9.87%,两组孕妇载脂蛋白C3第3 175位基因型频率比较,差异无统计学意义(P>0.05)。妊娠期高血压疾病组孕妇载脂蛋白C3基因第3 175位等位基因频率C、G分别为67.26%、32.74%,正常孕妇组孕妇分别为72.22%、27.78%,两组孕妇载脂蛋白C3基因第3 175位等位基因频率比较,差异无统计学意义(P>0.05)。②妊娠期高血压疾病组由轻到重的妊娠期高血压亚组、子痫前期-轻度亚组、子痫前期-重度亚组、子痫亚组孕妇载脂蛋白C3第3 175位等位基因频率比较,差异无统计学意义(P>0.05)。③比较妊娠期高血压疾病组载脂蛋白C3基因第3 175位CC、GC、GG 3种基因型孕妇血压升高程度及尿蛋白阳性率,差异无统计学意义(P值均>0.05)。结论:载脂蛋白C3基因第3 175位SNP与妊娠期高血压疾病发病无相关性,与妊娠期高血压疾病严重程度、血压升高程度和蛋白尿阳性率无相关性。

Objective： To explore the relationship between mononucleotide polymorphism of human apolipoprotein C3 （apo C3 ） gene at locus 3 175 （C→G） and pathogenesis of hypertensive disorder complicating pregnancy （HDCP） . Methods： The genotype of Sst I at locus 3 175 of human apo C3 gene from 84 cases with HDCP （HDCP group, including 28 cases of gestational hypertension, 13 cases of mild preeclampsia, 32 cases of severe preeelampsia, 11 cases of eclampsia） and 81 healthy pregnant women in late pregnancy （control group） were detected by polymerase chain reaction - restriction fragment length polymorphism （PCR - RFLP） . Results： （1）The frequencies of CC, GC and GG genotypes of apo C3 gene at locus 3 175 were 48.81% , 36. 90% and 14. 29% in HDCP group and 54. 32% , 35.80% and 9. 87% in control group, respectively （ P 〉 0. 05 ） . The frequencies of the allele C and allele G were 67. 26% , 32.74% in HDCP group, and 72. 22%, 27. 78% in control group, respectively （P 〉0. 05） . （2）There was no significant difference in allele frequency of apo C3 gene at locus 3 175 between different subgroups in HDCP group （ P 〉 0.05 ） . （3）There was no difference in elevation extent of blood pressure and positive rate of urine proteins between CC genotype, GC genotype and CG genotype of apo C3 gene at locus 3 175 in HD- CP group （P 〉 0.05 ） . Conclusion： There was no correlation between mononucleotide polymorphism of apo C3 gene at locus 3 175 and pathogenesis, degrees of HDCP, elevation extent of blood pressure, positive rate of urine proteins.