RhoC基因对宫颈癌细胞粘附和侵袭能力的影响

RhoC siRNA inhibits adhension and invasion of human cervical cancer Siha cell line in vitro

目的:研究RhoC对宫颈癌细胞粘附、侵袭和迁移能力的影响。方法:以宫颈癌SiHa细胞株为研究对象,转入RhoC小干扰RNA抑制其表达,通过体外粘附实验、侵袭实验和迁移试验比较SiHa细胞粘附率、侵袭能力和迁移能力的变化。结果:转入RhoC小干扰RNA后,RhoC蛋白的表达明显下调,对细胞外基质的粘附率明显下降(P<0.01),体外侵袭能力和迁移能力明显下降,侵袭抑制率和迁移抑制率分别为(19.94±6.06)%和(19.16±5.26)%。结论:RhoC能明显促进宫颈癌的转移。

Objective： To investigate the roles of RhoC to adhension, invasion and migration of cervical cancer. Methods： To transfect RhoC siRNA to downregulate the expression of RhoC. To test the adhensive ratio, invasive capability an migratory capability by adhesive assay, invasive assay and migratory assay. Results： Expression of RhoC was downregulated significantly after the RhoC siRNA was transfeeted. The adhesive ratio was decrease （P 〈 0. 01 ） . The invasive capability and migratory capability were depressed. The invasive inhibition ratio was （19. 94 ± 6. 06）% and the migratory inhibition ratio was （19. 16 ± 5.26）%. Conclusion： RhoC facilitates metastasis of cervical cancer.