ESBLs在产16S rRNA甲基化酶鸡大肠杆菌中的流行

Prevalence of ESBLs among 16S rRNA Methylase-producing Escherichia Coli Strains Isolated from Diseased Chickens

为了解河南地区超广谱β-内酰胺酶（ESBLs）耐药基因（TEM，SHV，OXA和CTX—M）在产16S rRNA甲基化酶鸡源大肠杆菌的流行，分别设计特异性引物对2005—2008年在河南地区病鸡的病料中分离保存的158株16S rRNA甲基化酶阳性鸡源大肠杆菌进行聚合酶链式反应扩增。检测结果显示，在158株产16S rRNA甲基化酶鸡大肠杆菌中，TEM，SHV，OXA和CTX—M检出率分别为94．3％，2.5％，62％和47．5％。在5组CTX-M组型中，CTX—M-2组型53株，CTX—M-9组型42株，CTX—M-2＆CTX—M-9组型20株。提示ESBLs在河南地区产16S rRNA甲基化酶鸡源大肠杆菌中普遍存在，且以TEM，OXA和CTX—M为主。ESBLs和氨基糖苷类高水平耐药16S rRNA甲基化酶基因同时出现在临床分离株中，加重了兽医临床治疗中抗菌药物选择的困难。

In order to investigate the prevalence of extended spectrum beta - lactamas （ESBLs： TEM, SHV, OXA and CTX - M） among 16S rRNA methylase - producing Escherichia coli strains isolated from dis- eased chickens in Henan Province, 158 16S rRNA methylase -producing E. coli strains isolated from diseased chickens during the years 2005 - 2008 were subjected to PCR for the presence of ESBLs. The results revealed ： of 158 16S rRNA methylase-producing E. coil strains, the positive rates for TEM,SHV, OXA and CTX-M were 94.3%, 2.5%, 62% and 47.5%, respectively, suggesting ESBLs were prevalent among 16S rRNA methylase -producing E. coli strains isolated from diseased chickens in Henan Province, with TEM, OXA and CTX - M dominant. Co - prevalence of ESBLs and high level aminoglycoside - resistant 16S rRNA methylase among clinical isolates would certainly aggravate the difficulties in drug choice for treatment in veterinary clinical practice.