江西三清山华东黄杉种群遗传多样性研究

ISSR Analysis of the Genetic Diversity of Pseudotsuga gaussenii Flous of Sanqing Mountain in Jiangxi Province

采用ISSR分子标记技术分析江西三清山5个华东黄杉居群的遗传多样性水平和居群遗传结构。用10个引物对5个居群共45个样品进行扩增，共得到76条清晰的扩增带，其中68个位点为多态性位点。在物种水平上，多态性百分率（PPB）为89．47％，Nei＇s基因多样性指数（H）为0．2827，Shannon＇s信息多样性指数（I）为0．4322。在居群水平上，多态性位点百分率在56．58％-85．53％，Nei＇s基因多样性指数为0．1913～0．2827，Shannon＇s信息多样性指数为0．2905—0．4322。物种和居群遗传多样性水平都较高，居群间产生了一定的遗传分化（Gst=0．2090，Фst=14．05％）。由UPGMA聚类分析可知，1（SGTH）和2（LQJ）两个居群优先聚类，4（LM）和5（YJF）聚为一支，居群的亲缘关系与地理距离及海拔高度有一定的相关性。

Pseudotsuga gaussenii Flous were collected from Sanqing Mountain in Jiangxi Province, and their genetic diversity and genetic differentiation were analyzed by using inter - simple sequence repeat （ ISSR） molecular technique. ISSR amplification was conducted with 10 primers for 45 individuals from 5 populations, and 68 polymorphic loci were detected from 76 loci, indicating high genetic variation at species level （ PPB = 89.47 %, H = 0. 2827, I = 0.4322） and at population level （PPB = 56. 58% - 85. 53 %,H = 0. 191 3 - 0. 282 7, I = 0.290 5 - 0.432 2）. A certain level of genetic differentiation occurred among populations （ Gst = 0. 209 0, Фst = 14.05% ）. The dendrograms of genetic relationships among populations were constructed based on Nei＇ s genetic distance. The 1 （SGTH） and 2 （LQJ） populations clustered in a clade, the 4 （LM） and 5 （YJF） populations clustered in another clade too. The result shows that genetic distance is related with geographic distance and altitude.