摘要
从阴沟肠杆菌(Enterobacter cloacae)UW4菌株克隆出ACC脱氨酶(1-aminocyclopropane-1-carboxylicacid deaminase)基因连接至pGEM-Tvector上,并采用3种不同启动子(组成型表达启动子CaMV35S、花特异表达启动子CHSA及衰老特异表达启动子SAG12)分别构建ACC脱氨酶基因的植物表达载体,经PCR及酶切鉴定均已构建成功,为后期选择不同的花卉等植物材料遗传转化研究打下基础。
ACC deaminase gene was cloned from Enterobacter cloacae UW4 strain and ligated into pGEM -T vector. Three different promoters (constitutive expression promoter CaMV35S, flower specific expression promoter CHSA and senescence specific expression promoter SAG12) were respectively utilized to successfully construct three plant expression vectors of ACC deaminase gene after PCR and restriction endonucleases detection, which laid the foundation for the future part of selecting proper flower plants and carrying out its genetic transformation.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2009年第4期706-710,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金项目(30560095)
云南省自然科学基金项目(2005C0004M)
西南林学院基金项目(200522M)
关键词
ACC脱氨酶基因
花特异表达启动子
衰老特异表达启动子
植物表达载体构建
ACC deaminase gene
flower specific expression promoter CHSA
senescence specific expression promoter SAG12
plant expression vector construction