地衣芽孢杆菌L3发酵培养基的响应面法优化

Optimization of Bacillus licheniformis L3 Fermentation Medium by Response Surface Method

地衣芽孢杆菌L3对镰刀菌、核盘菌等显示出强的抑菌活性,对其发酵培养基进行了优化研究。在单因素试验的基础上,采用Plackett-Burman设计确定了碳源、豆饼粉和K2HPO4等显著影响芽孢形成和菌体数量的因素。通过最陡爬坡路径试验、综合中心组合设计和响应面分析法得出最优培养基组成为豆饼粉8.0g·L-1、K2HPO42.00g·L-1,碳源32.2g·L-1,MgSO40.5g·L-1,酵母膏2.5g·L-1,地衣芽孢杆菌L3的活菌体数目可以达到100.20×108CFU·mL-1,比初始培养基提高近1倍。

Bacillus licheniformis L3 showed strong fungicidal activity against Fusarium spp. and Sclerotinia spp. Based on the single-factor test, carbon source, soybean powder and K2HPO4 were determined as necessary for spore formation of Bacillus licheniformis L3 through the Plackett-Burman design. The medium consist with soybean flour 8 g·L^-1, K2HPO4 2 g·L^-1,carbon 32.20g·L^-1, MgSO4 0.5g·L^-1, yeast extract 2.5 g·L^-1 were defined as the optimum medium for Bacillus licheniformis L3 growth and spore formation, through the center combination of design and response surface analysis. With the optimum medium the live bacteria number was 100.20×10^8CFU·mL^-1, nearly double than that with the initial culture medium.