三七总皂甙对不同时间尿激酶溶栓出血性转化的干预研究

Study of Panax Notoginseng Saponins on Thrombolysis-induced Hemorrhagic Transformation in Rats with Thrombusoccluded Cerebral Ischemia

目的观察三七总皂甙(PNS)抑制基质金属蛋白酶-9(MMP-9)对大鼠缺血性脑卒中不同时间尿激酶溶栓后血脑屏障破坏和颅内出血性转化的干预效果,探寻减轻溶栓并发症,延长溶栓时间窗的途径。方法采用大鼠自体血栓栓塞大脑中动脉闭塞(MCAO)模型,脑组织中MMP-9表达采用免疫组织化学法,血脑屏障通透性采用伊文氏蓝法,脑出血量的测定采用分光光度计法。结果各时间点尿激酶溶栓组MMP-9表达和脑出血量明显增加,血脑屏障通透性也有增加趋势,随溶栓时间延迟上述指标增加越明显;PNS联合治疗组与单用尿激酶组相应时间点比较MMP-9表达显著下降,血脑屏障通透性和脑出血量均有改善。结论PNS可抑制溶栓后MMP-9高表达,减轻溶栓后血脑屏障破坏和颅内出血性转化,减轻溶栓并发症。

Objective To investigate the effects of panax notoginseng saponms （PNS） on hemorrhage transformation and blood-brain barrier opening. Methods Middle eerehral attery occlusion（MCAO） rats were established by embolizing left middle cerebral artery with autologous blood clots, Tbe expression of matrix metallopeptidasc- 9 （MMP- 9）was detected with immunohistochemieal staining, the permeability of the brain blood barrier was obserw：d with Evan＇s blue and the cerebral hemorrhage was quantified with spectrophotometer assay. Results MMP - 9 expression,cerebral hemorrhage content and the permeability of the brain -blood barrier were increased with urokinase treatment. And there were obviously increased at more than 6 hour after ischemic stroke. After treatment of urokinase combined with panax notoginseng saponins, the expression of MMP 9 was decreased,and thrombolysis （urokinase）- induced hemorrhage and the brain - blood barrier disruption were reduced. Conclusiun Panax notoginseng saponins could attenuate urokinase - induced the expression of MMP - 9,tbe breakdown of brain blood barri and the cerebral hemorrhage content.