止颤汤对帕金森病大鼠神经干细胞诱导分化作用的研究

Study of the Inducing Effect of Zhichan Soup on the Neuron Stem Cell Differentiation of Parkinson's Disease Model Rats

目的:探讨止颤汤对帕金森病大鼠神经干细胞的增殖及向多巴胺能神经元分化的作用;方法:实验动物分为3组,正常组及模型组用生理盐水灌胃,止颤汤组用止颤汤灌胃,分别在处理后7、14、28天免疫组化法检测,神经干细胞标志物nestin及其在脑内位置变化,多巴胺递质转运子DAT蛋白表达。比较各组差异;结果:在动物处理后28天,止颤汤组帕金森病模型大鼠脑内nestin及DAT数量较对照组增多,且多分布在中脑损伤区域;结论:止颤汤对帕金森病大鼠脑内神经干细胞的增殖及向多巴胺能神经元分化有一定促进作用。

Objective： To study the inducing effect of Zhichan Soup on the neuron stem cell differentiation of Parkinson＇s disease model rats; Methods： the rats were divided into three groups, the normal and model groups were administered with 0.9% normal saline by garage ,and the Zhichan Soup group with Zhichan Soup,the nestin and DAT protein were checked with immunohistochemical method respectively after the rats were treated 7,14 and 28 days .Results： compared to the control group, the nestin and DAT protein were significantly increased in the Zhichan Soup group at the 28 days time point, and the nestin protein were mainly appeared in midbrain.Conclusion： Zhichan Soup has the effect to promote the proliferation and differentiation of the of neuron stem cell Parkinson＇s disease model rats.