玉米和花生中黄曲霉素B_1酶联免疫快速分析方法

Enzyme Immunoassay of Aflatoxin B 1 in Corn and Peanut.

将黄曲霉素Ｂ１衍生成胺氧乙酸肟，然后分别同牛血清白蛋白和辣根过氧化物酶进行化学交联而制得结合物。前者用来免疫新西兰大白兔，后者用作免疫反应的示踪物。将所得抗体和示踪物在免疫反应中进行浓度优化，开发出了ＡＦＢ１酶联免疫分析直接竞争抑制法。用所建立的方法能够准确、快速地检测玉米和花生中黄曲霉素Ｂ１的含量。ＡＦＢ１在磷酸盐－吐温缓冲液中的最低检测限为０．４ｐｇ／ｍＬ；在玉米和花生中的最低检测限均为４０ｎｇ／ｋｇ。

An enzyme immunoassay (EIA) was developed for aflatoxin B 1 in corn and peanut. The EIA was based on antibodies raised against the oxime derivative of AFB 1 by immunizing rabbits with a bovine serum albumin AFB 1 oxime conjugate. Horse-radish peroxidase was conjugated to AFB 1 by water soluble 1-[3-(dimethyl-aminopropyl]-3-ethylcarbodimide, the conjugate was used as a tracer in immuno-reaction. With the method developed, the minimum detection limits for were 0.4 pg/mL in phosphate-buffed saline with Tween-20, and 40 ng/kg in corn and peanut.