供水管网不同管材内壁微生物分布的显微观察

Microscope Observation of the Microbial Distribution on the Wall of Different Pipe Material in Water Supply System

采用扫描电镜、原子力显微镜以及激光共聚焦显微镜对不锈钢插片表面和供水管网末端PPR管内壁微生物的分布和生物膜结构进行了观察和分析.结果表明,在供水管网中,不锈钢材质表面不利于微生物附着生长,在短时间内(3个月)只有很少的微生物附着.在管网末端PPR管表面有明显的微生物附着生长,细菌容易附着在基底表面的凹穴中,细菌与基底是紧密黏附在一起的.扫描电镜和原子力显微镜结合是观察管壁非连续生长生物膜表面结构的有效手段.采用激光扫描共聚焦显微镜可同时实现管壁生物膜内部和三维结构观察.

Scanning electron microscope, atomic force microscope and confocal laser scanning microscope were used to observe the microbial distribution and biofilm structure on the surface of stainless steel plugs and the wall of PPR pipe in the water distribution system. The results show that, it is not easy for microbial to attach on the surface of stainless steel plugs. Little bacteria was observed in a short period of time（less than 3 months）. Bacteria can be easily observed in the sediment of the PPR pipe wall at the end of water supply system. Bacteria was tightly adhered to the surface of the PPR pipe wall. Combination of scanning electron microscope and atomic force microscope is a practical way to observe the surface structure of discontinuous biofilm in the pipeline. The inner and three-dimensional structure of the pipe biofilm can be simultaneously observed by confocal laser scanning microscope.