利用邻苯二甲酸二甲酯培养好氧颗粒污泥研究

Aerobic Granulation for Dimethyl Phthalate Biodegradation in a Sequencing Batch Reactor

以活性污泥为接种污泥,葡萄糖为共代谢基质,通过控制有机负荷、污泥沉降时间,在序批式反应器(sequencing batchreactor,SBR)内培养得到具有邻苯二甲酸二甲酯(dimethyl phthalate,DMP)降解性能的好氧颗粒污泥.结果表明,好氧颗粒污泥对DMP具有很好的去除效果,驯化75 d后,DMP与COD去除率分别达到92.3%与90.6%.好氧颗粒污泥降解DMP的中间产物为邻苯二甲酸单酯(monomethyl phthalate,MMP)和邻苯二甲酸(phthalic acid,PA).动力学研究表明,DMP降解过程符合haldane抑制模型,Vmax为643.2 mg.(g.h)-1,Ks和Ki分别171.0 mg.L-1和337.5 mg.L-1.电镜观察表明,颗粒污泥表面粗糙,微生物相丰富,含有丝状菌、球菌和短杆菌.

This study investigated the feasibility of aerobic granulation for dimethyl phthalate （DMP） with the glucose as co-metabolism substrate in a sequencing batch reactor （SBR）. Experimental results showed that aerobic granulation could be successfully realized in the reactor by controlling the organic loading and sludge settling time. The system could achieve average removal efficiencies of 92.3 % for DMP and 90.6 % for COD after 75 days operation. The DMP intermediate metabolites were determined and identified as monomethyl phthalate and phthalic acid. Degradation kinetics was well described by substrate inhibition pattern as Haldane equation, and the corresponding kinetic coefficients of V Ks and Ki were 643.2 mg·（g·h）^-1, 171.0 mg·L^-1 and 337.5 mg·L^-1, respectively. Environmental scanning electronic microscope （ESEM） results showed that a clearly defined boundary with high porosity on the coarse surface. Many filamentous, coccoid and rod-shape bacteria were visible on the external surface of the granules.