三疣梭子蟹4个野生群体遗传差异的同工酶分析

Genetic variation analysis of four wild populations of Portunus trituberculatus by isozyme

采用聚丙烯酰胺不连续凝胶垂直电泳技术对三疣梭子蟹Portunus trituberbuculatus4个野生群体(鸭绿江口、莱州湾、海州湾和舟山)的同工酶进行检测,分析群体间的生化遗传差异。结果发现(1)4个群体的多态座位百分数(P0.99)分别为:鸭绿江口13.6%、莱州湾13.6%、海州湾13.6%和舟山13.6%。(2)4个群体间的生化遗传差异不显著,4个群体间的遗传距离D=0.00054～0.00170,属种内群体间差异。(3)聚类分析表明:4个群体分为两组,海州湾和舟山两群体之间的亲缘关系最近,聚为一组。莱州湾和鸭绿江口群体亲缘关系比较近,聚为另一组。

Vertical polyacrylamide gel electrophoresis was used to investigate the genetic var- iation of isozyme of Portunus trituberbuculatus. Four wild geographic populations distributed in the Yalujiangkou（YL）, Laizhou Bay（LZ）, Zhoushan （ZS） and Haizhou Bay（HZ） （48 individuals each ）were surveyed. Of 22 loci encoding 11 enzymes, three were polymorphic. The results showed that. （1）the mean proportion of polymorphic loci（P0.99） of YL, LZ, ZS and HZ were 13.6 %, 13.6 %, 13.6 % and 13.6 %, respectively ; （2） there were no significant differences in biochemical genetic characteristics of the P. trituberbuculatus among four wild geographic popula- tions. The genetic divergences （Dnei） among the four wild geographic populations ranged from 0. 00054 to 0. 00170, which were lower than that between subspecies; （3） dendrogram construc- ted based on the genetic distances among the four populations showed that there were two different groups, one being composed of YL and LZ and the other of ZS and HZ.