消毒剂对金黄色葡萄球菌和大肠杆菌诱导L型形成的实验观察

EXPERIMENTAL OBSERVATION ON INDUCTION OF L-FORM FORMATION OF STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI BY DISINFECTANTS

以不同浓度的过氧化氢、碘伏、乙醇、苯扎溴铵和醋酸氯己定作用于金黄色葡萄球菌和大肠杆菌１～１０ｍｉｎ，仅７５％乙醇作用１ｍｉｎ可诱导金黄色葡萄球菌形成Ｌ型。其菌落多为“油煎蛋”样，少数呈较小的颗粒状。ＤＮＡ酶活性、溶血作用、发酵部分糖与甘露醇及产生金黄色色素能力丧失，但凝固酶仍为阳性。

taphylococcus aureus and Escherichia coli were exposed to hydrogen peroxide, iodophor, ethanol, neogeramine, chlorhexidine acetate at different concentrations for 1～10 min The results showed that 75% ethanol with a 1 min contact time could induce L-form formation of Staphylococcus aureus, while 25% and 50% ethanol with a 1～10 min contact time and other 4 disinfectants could not induce L-form formation of S aureus and all the five disinfectants could not induce L-form formation of Escherichia coliThe colonies of L-form of S aureus were mostly 'fried egg' -like and a few appeared as small granules The L-form lost its DNAase activity, hemolytic action and the power of fermenting mannitol, sucrose and trehalose and producing golden-yellow pigment, but coagulase test was still positive