胃泌素体外促大肠癌细胞分泌表皮生长因子的实验研究

The Effect of Gastrin in Increasing Epidermal Growth Factor in the Development of Colorectal Cancer in Vitro

目的:探讨胃泌素和表皮生长因子在大肠息肉、大肠肿瘤发生发展中的作用。方法:取大肠息肉和肿瘤组织体外培养并用五肽胃泌素及丙谷胺干预,用放免法检测表皮生长因子(EGF)含量并分析其意义。结果:大肠肿瘤组织中胃泌素组EGF含量明显高于空白对照组;腺瘤组织中胃泌素组EGF含量明显高于空白对照组及胃泌素+丙谷胺组。结论:胃泌素能够促进体外培养大肠肿瘤及腺瘤组织中EGF的分泌,从而促进肿瘤生长,丙谷胺可部分抑制其作用。

Objective：To investigate the effect of gastrin and epidermal growth factor（EGF） in the development of colorectal ployp and cancer. Methods： The tissue of colorectal ployp and cancer was cultured in vitro and interfered with pentagastrin （PG） and proglumide （PGL）. EGF was detected by radioimmunoassay, and then its significance was determined. Results： In the tissue of colorectal cancer, the content of EGF in PG group was more than that in control group. In the tissue of colorectal adenoma, the content of EGF in PG group was more than that in control group and PG plus PGL group, Conclusion： Pentagastrin can stimulate the secretion of EGF in the tissue of colorectal cancer and adenoma in vitro, thereby promote the growth of it. The proglumide has some inhibition.