运脾方对小鼠血清溶血素抗体生成作用的影响

Study of Yun-Pi Prescription on Serum Hemolysin Antibody of Mice

目的观察运脾方对小鼠溶血素抗体生成作用的影响,探讨其提高免疫功能的作用机理。方法健康KM小鼠48只,随机分成正常对照组及运脾方大、中、小剂量组,正常对照组灌服蒸馏水,运脾方大、中、小剂量组分别灌服20、10、5mL/kg运脾方糖浆制剂,连续给药7d。第7日给每只小鼠腹腔注射5%鸡红细胞(CRBC)混悬液0.2mL进行免疫,连续7d。检测血清溶血光密度值。结果运脾方大、中、小剂量组分别与正常对照组比较,小鼠的血清溶血光密度值明显增加(P<0.001)。结论运脾方提高免疫功能的作用机理与增加血清溶血素抗体水平密切相关。

Objective To investigate the effects of Yun-Pi Prescription on hemolysin antibody, and approach its mechanism of regulating immune function. Methods Forty-eight KM mice were randomly divided into high, medium and low dose of Yun-Pi Prescription groups, which were given 20, 10, 5 mL/kg Yun-Pi Prescription respectively, and one control group with no intervention. From the seventh day, all groups were injected with chicken red blood cell for seven days. Levels of serum hemolytic optical density were measured in all groups. Results Compared with control group, levels of serum hemolytic optical density in Yun-Pi Prescription groups increased significantly （P〈0.001）. Conclusion Yun-Pi Prescription remarkably enhanced immune function, which has closely relation to increasing mice levels of serum hemolysin antibody.