针刺对肺癌大鼠体内紫杉醇脂质体趋向性的影响

Effects of Acupuncture on Paclitaxel Liposome Tropism of Tumor Organization in Vivo in Lung Cancer Rats

目的观察针刺"肺俞"、"太渊"对紫杉醇脂质体在肺癌大鼠体内趋向性的影响,为抗肿瘤药物的靶向性研究开辟新的途径。方法紫杉醇脂质体用99m锝标记,选用单纯尾静脉注射药物以及注射药物分别与针刺"肺俞"、"太渊"(相关穴位)、"神门"(无关穴位)相结合等4种方法对造模成功的肺癌大鼠进行干预(每组18只)。分别于静脉注射后60、90、120min(每个时间点取6只)后处死大鼠,用γ-放射免疫记数器分别测定肺、肝、肾等脏器紫杉醇脂质体的药物含量。结果针刺"肺俞"和"太渊"在全部或部分时间点使肺癌大鼠肺组织紫杉醇脂质体含量增加。针刺"神门"未能使肺组织药物含量升高。结论针刺"肺俞"和"太渊"对紫杉醇脂质体在肺癌大鼠体内的分布具有趋向性影响,"肺俞"对药物在肺脏的靶向性影响大于"太渊"。

Objective To observe the effect on body distribution tendency of paclitaxel liposome in the lung cancer rats after acupuncture of Feishu, Taiyuan acupoints in order to develop the new path for the target study of anticancer drugs. Method The paclitaxel liposome was labeled by technetium （Tc）. Four methods including tail intravenous injection of paclitaxel liposome merely and the combination of tail intravenous injection of paclitaxel liposome with acupuncture of Feishu, Taiyuan （concerned acupoint） and Shenmen （unconcerned acupoint） acupoints respectively were carried on the intervention to the lung cancer rat models （18 in each group）. The rats were sentenced to death at 60, 90, 120 minutes （6 at each time point） respectively after drug injection, and the drug content of paclitaxel liposome in the lung, liver, kidney organs were measureed by the γ-radiate immunity counting implement respectively. Result Acupuncturing at Feishu and Taiyuan can lead to the drug content increment of paclitaxel liposome in the lung organization of the lung cancer rats in all or parts of time points. On the contrary, acupuncturing at Shenmen can not. Conclusion Acupuncturing Feishu and Taiyuan can make tropism effect on the disposition of paclitaxel liposome in vivo in lung cancer rats. Feishu is stronger than Taiyuan on the drug tropism of the lung.