HPLC比较不同产地生药八角莲中鬼臼类成分含量

Comparison of Podophyllotoxin and 4’-Demethylpodophyllotoxin Contents in Rhizoma et Radix Dysosmae from Different Areas by HPLC

目的比较不同产地的八角莲中鬼臼毒素和4’-去甲鬼臼毒素含量。方法采用RP-HPLC,色谱柱C18(250mm×4.6mm,5μm),流动相为甲醇-0.1mol·L-1K2HPO4(50∶50),流速1.0mL·min-1,检测波长207nm,柱温25℃。结果鬼臼毒素的线性范围20～400ng,r=0.9999,回收率100.4%,RSD=1.2%;4’-去甲鬼臼毒素的线性范围2～20ng,r=0.9998,回收率101.6%,RSD=2.5%。结论该方法准确、可靠、简便,不同地域的生药八角莲中鬼臼类成分含量差异明显。

OBJECTIVE To compare the contents of podophyllotoxin and 4’-demethylpodophyllotoxin in Rhizoma et Radix Dysosmae of different areas. METHODS The assay was performed on phenomenex C18 （250 mm×4.6 mm, 5 μm）. The mobile phase was MeOH-0.1 mol？L^-1K2HPO4（50∶50）. Flow rate was 1.0 mL？min^-1. Wavelength was 207 nm, and the temperature of column was 25 ℃. RESULTS The calibration curve was linear in the range of 20-400 ng for podophyllotoxin （r=0.999 9）. The average recovery was 100.4% （RSD=1.2%）. And the liner range of demethylpodophyllotoxin was 2-20 ng （r=0.999 8）. The recovery was 101.6%（RSD=2.5%）. CONCLUSION The method is accurate, reliable and simple, the apparent differences of contents among samples was found.