三七总皂甙对血清及肺组织血管紧张素转换酶的影响

EFFECTS OF PANAX NOTOGINSENG SAPONINS ON ANGIOTENSIN CONVERTING ENZYMES IN SERUM AND LUNG TISSUE

目的：观察三七总皂甙（ＰＮＳ）对血清和肺组织血管紧张素转换酶（ＡＣＥ）的影响。方法：①ＰＮＳ与马尿酰甘氨酰甘氨酸和血清或肺组织匀浆共浴；②静脉注射ＰＮＳ，应用紫外分光光度法测定马尿酸产出率，以其代表ＡＣＥ活性。结果：离体反应显示，随着ＰＮＳ浓度增高（０．１５～１．２ｍｇ／ｍｌ），大白鼠血清、肺组织和人血清ＡＣＥ活性逐渐下降（Ｐ＜０．０１）。ＰＮＳ为１．２ｍｇ／ｍｌ时，抑制率分别为（６１．５４±１１．７６）％、（７２．５４±７．８３）％和（６２．０９±１６．４）％，三者之间无显著性差异（Ｐ＞０．０５）。ＰＮＳ（７５ｍｇ／ｋｇ）静脉注射家兔血清ＡＣＥ活性从（６２．５２±１２．４３）ＩＵ下降为（４４．８０±１０．４７）ＩＵ（Ｐ＜０．００１）。结论：ＰＮＳ对循环和局部组织ＡＣＥ活性均有抑制作用。

Objective:To investigate the effect of panax notoginseng saponins (PNS) on angiotensin converting enzyme (ACE) activity Methods:①PNS, hippuryl glycylglycing and serum or lung tissue homogenate were mixed and incubated in vitro ②PNS was injected intravenously The ACE activity was determined by measuringthe hippuric acid generated from hippuryl glycylglycing, the substrate, with a UV spectrophotometer Results: PNS at 0 15￣1 2 mg/ml inhibited the ACE activity in a concentration dependent manner The inhibition of ACE by PNS at 1 2 mg/ml in the rat serum, the rat lung tissue homogenate and the human serum were (61 54±11 76)%,(72 54±7 83)% and (62 09±16 43)%, respectively It is no significient difference between these three (P>0 05) At 75 mg/kg, PNS decreased the sernm ACE activity in rabbit from (62 52±12 43)IU to (44 80±10 47)IU Conclusion: PNS has an inhibition effect on the activities of ACE in circulation and local tissue The mechanism of the inhibition effect of PNS needs to be futher investigated and might be defferent from that of the synthetic ACE inhibitors