鼻咽癌P^(53),C-myc,EGFR基因表达的研究

STUDIES ON P 53 , C myc, EGFR GENES EXPRESSIONS IN NASOPHARYNGEAL CARCINOMA

目的：Ｐ５３，Ｃ－ｍｙｃ及ＥＧＦＲ基因在鼻咽癌（ＮＰＣ）的发生、发展中可能起重要作用，基因的研究结果可能为ＮＰＣ的预防及诊疗提供科学的依据。方法：用免疫组化Ｓ－Ｐ法检测了３０例ＮＰＣ组织、１０例无瘤鼻咽部（ＮＰ）组织的Ｐ５３，Ｃ－ｍｙｃ及ＥＧＦＲ基因的异常表达情况。结果：①三种基因在ＮＰＣ组织中均有较高水平的表达，Ｐ５３蛋白阳性率为８６．７％（２６／３０），Ｃ－ｍｙｃ和ＥＧＦＲ蛋白阳性率均为７０％（２１／３０）；相应对照的无瘤ＮＰ组织的阳性率分别为０．０％（０／１０）、１０％（１／１０）和３０％（３／１０）。ＮＰＣ组织与无瘤ＮＰ组织的Ｐ５３，Ｃ－ｍｙｃ及ＥＧＦＲ蛋白阳性率高于无瘤ＮＰ组织相应的阳性率，差异有统计学意义（Ｐ＝０．００００、Ｐ＝０．００１３及Ｐ＝０．０３１８）；②ＮＰＣ按ＴＮＭ临床分期，将其Ｔ、Ｎ级数分别与Ｐ５３、Ｃ－ｍｙｃ，ＥＧＦＲ蛋白染色阳性程度进行相关分析，皆无统计学意义（Ｐ＞０．０５）；③Ｃ－ｍｙｃ与ＥＧＦＲ的阳性表达之间有相关性（Ｐ＝０．０００９）。结论：①Ｐ５３，Ｃ－ｍｙｃ，ＥＧＦＲ三种基因的高表达，说明其与ＮＰＣ的发生有一定关系，认为Ｐ５３的免疫组化检测可作为ＮＰＣ的辅助诊断依据；?

Objective:Genes of P 53 , C myc and EGFR probably play an important roles in the genesis and develop ment of NPC.Studies on these genes may provide some scientific basis for the prevention,diagnosis and treatment of NPC.Methods:30 cases of nasopharyngeal carcinomas (NPC) and 10 cases of tumor free nasopharyngeal mucosae(control group) were detected for expressions of P 53 , C myc, EGFR by immunohistochemistry (IHC) S P technique.Results:① All the genes highly expressed in NPC.Positive percentages were P 53 86 7% (26/30), C myc 70%(21/30),EGFR 70%(21/30), while positive rates of the control group were 0 0% (0/10), 10%(1/10) and 30%(3/10) respectively.Rates comparison between groups revealed significant difference (P=0 000 0,P=0 001 3 and P=0 031 8 respectively).②No significant difference was found in correlation analysis of NPC T or N stages with staining degree of P 53 , C myc and EGFR proteins (P>0 05). ③ Expression correlated significantly (P=0 000 9)between of C myc and EGFR.Conclusion:① The three genes play some roles in the genesis of NPC according to their high expressions in NPC.The result of P 53 IHC examination is helpful in NPC diagnosis. ② There is no statistical correlation between the expressions of the three genes and T,N stages of NPC. ③ The correlation of C myc and EGFR indicates their cooperation in NPC oncogensis.