桃SRAP体系的优化及与SSR在桃品种鉴定上的比较

Peach SRAP System Optimization and SSR Peach Varieties in the Comparative Identification

为确立的桃10μL SRAP反应体系,以桃部分品种为试材,采用均匀设计,对SRAP-PCR反应体系中TaqDNA聚合酶、模板DNA、dNTPs、Mg2+、引物5个组分的浓度进行优化。结果表明,桃10μL的SRAP反应体系的最佳组分包括2 UTaqDNA聚合酶5、0 ng模板DNA、0.6 mmol/L dNTPs0、.25μmol/L引物、2.0μL 10×PCR Buffer+Mg2+。利用所确立的体系对其他部分桃种质进行扩增的结果清晰可靠,多态性好。并且利用随机挑选的SRAP引物和SSR引物分别区分鉴定8种桃种质,发现SRAP用5对可以将桃种质区分开,而SSR用13对才区分开,说明在桃品种鉴定上SRAP比SSR更省时方便,而且操作相对简单,适用性强。

The Peach some varieties were used to adopt uniform design,the SRAP-PCR reaction system Taq DNA polymerase, the template DNA, dNTPs, Mg^2＋ , primers five components to optimize the concentration. The results showed that 10 g L Peach SRAP reaction system of the best components,including 2.5 μL 10 × PCR Buffer ＋ Mg^2＋ , 1U Taq DNA polymerase,40 ng template DNA,0.2 mmol/L dNTPs,0.2 μmol/L primer. Established by the use of the system to other parts of peach germplasm to amplify the results of clear and reliable, good polymorphism. And using random selection of the SRAP primer and SSR primer respectively distinction between identification of eight kinds of peach germplasm and found that SRAP can be used to separate peach germplasm, and the SSR was used to separate, that the peach variety identification on the SRAP in more than SSR Convenient, and relatively simple operation, the applicability.