肝癌切除手术前后周围静脉血液中AFPmRNA的变化及其意义

Influence of hepatectomy for hepatocellular carcinoma on tumor cells spreading in blood.

目的探讨肝癌切除手术前后周围静脉血液中AFPmRNA的变化及其意义。方法应用巢式RT-PCR检测术后48小时、72小时及术后1周肝癌患者周围静脉血液中AFPmRNA,并观察变化。结果28例术前血液中AFPmRNA阴性的肝癌患者中有4例术后48小时转为阳性(14.30%),但术后1周时复转阴性;而16例术前血液中AFPmRNA阳性的肝癌患者中有4例(25.00%)术后72小时转为阴性。结论手术操作可引起肿瘤细胞播散人血循环,但它不增加手术危险性。术后短期适量应用γ-干扰素或白介素2(IL-2)是必要的。

Objective To study the influence of hepatectomy for hepatocellular carcinoma (HCC) on tumor cells spreading in blood. Methods Using nested RT-PCRT, AFPm RNA content in 28 blood samples from the HCC patients were deterind and the changes observed 48 h, 72 h and one week after operation. Results In 28 patients with blood AFPmRNA being negative before operation, positive AFPmRNA was found in 4 cases 48 h after operation and disappeared one week after operation. Conclusion The operation can promote the tumor cells spreading into blood, which did not increase the danger of distant metastasis.