增殖细胞核抗原在肝硬化、肝细胞不典型增生和肝细胞癌中的表达及意义

Expression of proliferating cell nuclear antigen in cirrhosis, liver cell dysplasia and hepatocellular carcinoma.

目的探讨肝细胞癌(HCC)的分化状态与增殖细胞核抗原(PCNA)表达的关系。方法采用PAP酶标法半定量检测(PCNA)在19例肝硬变、21例肝细胞不典型增生(LCD)和54例HCC中的标记指数(LI)。结果 PCNA主要定位于肝癌细胞核中。在HCC Ⅰ级、Ⅱ级、Ⅲ级中PCNA的标记指数(LI)分别为20.6%、42.2%、68.9%,均存在着显著性差异(P<0.01);在肝硬变、LCD中的LI分别为8.8%、18.8%,两者间无显著性差异(P>0.05)。结论 HCC的分化程度与PCNA的LI密切相关,原位检测HCC组织中的PCNA表达,是判断HCC的分化程度的一种较为客观、简便的方法。

Objective To determine the cell proliferating status in three classes of specimens and investigate the relationship between hepatocellular carcinoma (HCC) differentiation and proliferating cell nuclear antigen (PCNA) expression. Methods A half quantitative analysis of the labeling indices (LI) using PAP enzyme labeling method was performed to determine the LI of PCNA expression in 19 patients with cirrhosis, 21 with liver cell dysplasia (LCD) and 54 with HCC. Results It was found that PCNA was mainly located in the cell nuclear of HCC. LI of PCNA at stags of Ⅰ, Ⅱ and Ⅲ of HCC was 20.6%, 42.2%, and 68.9%, respectively. Significant difference in the LI of PCNA was found among the various stages of HCC (P<0.01). The LI of PCNA in cirrhosis and LCD was 8.8% and 18.6% respectively without significant difference between them (P>0.05). Conclusion The differentiation of HCC is closely related with LI of PCNA. The determination of PCNA expression in HCC tissue is an objective and simple method for the prediction of HCC differentiation.