血管内皮生长因子在低氧性肺动脉高压大鼠肺内的分布和水平变化

Changes of Level and Distribution of Vascular EndothelialGrowth Factor in the Lungs of Rats withHypoxic Pulmonary Hypertension

为了解低氧性肺动脉高压时肺内血管内皮生长因子（ＶＥＧＦ）的改变及其意义，以常压低氧建立大鼠肺动脉高压模型，采用免疫组织化学染色法和Ｅｌｉｓａ法检测模型大鼠肺内ＶＥＧＦ的分布和含量变化。结果显示：低氧３周后大鼠形成明显的肺动脉高压；大鼠肺匀浆内ＶＥＧＦ含量在低氧３周组为４６６．９±７５．５ｐｇ／ｇ，明显高于对照组（３７６．２±４７．１ｐｇ／ｇ）；肺小动脉内膜的ＶＥＧＦ免疫组化染色阳性在低氧大鼠组明显增强。提示低氧在引起肺动脉高压的同时，可强烈刺激ＶＥＧＦ的合成和分泌，后者可能在低氧性肺血管重建过程中发挥重要的作用。

This study was designed to elucidate whether the level and distribution of vascular endothelial growth factor (VEGF) are changed in the lungs of rats with hypoxic pulmonary hypertension. 13 male Wistar rats were exposed to isobaric hypoxia for 3 weeks. The pulmonary artery pressure was measured by right cardiac catheterization. The level of VEGF in pulmonary homogenate was measured by Elisa method. The distribution of VEGF in the rat lung was examined by immunohistochemistry. The results showed that the pulmonary artery pressure was significantly increased after hypoxic exposure. The level of VEGF in pulmonary homogenate of rats treated with hypoxia (4669±755 pg/g) were significantly increased as compared with taht of normal rats (3762±471 pg/g). The contents of VEGF in the wall of pulmonary arteriole were significantly increased in rats with pulmonary hypertension. So we suggest that chronic hypoxia can strongly stimulate VEGF secretion, and VEGF may mediate the process of hypoxic pulmonary vascular remodeling and pulmonary hypertension.