大鼠肺动脉平滑肌细胞的分离及鉴定

Isolation and Identification of Smooth Muscle Cells from Pulmonary Artery in Rats

为探讨肺动脉平滑肌细胞的急性分离方法，在机械分离出大鼠肺动脉后，应用胶原酶和木瓜蛋白酶对大鼠肺动脉平滑肌细胞进行急性分离，并用电子显微镜对所分离出的细胞进行鉴定。结果成功地分离出单个、梭型的肺动脉平滑肌细胞，并经电镜观察证实分离液中９０％的细胞为肺动脉平滑肌细胞。该方法操作较简便，结果稳定，重复性好，可推广使用。

To explore the method of isolating acutely the smooth muscle cells from pulmonary artery in rats, small pulmonary arteries (700200 μm, ID) were dissected free of connective tissue and were allowed to digest in a N2hydroxyethylpiperazineN′2ethanesulfonic acid(HEPES)buffered physiological saline solution (HPSS) containing collagenase, papain and bovine serum albumin. The tissue was then triturated to disperse smooth muscle cells. The isolated cells in suspension were identified and photographed with film on electron microscope (EM). We succeeded in isolating the single smooth muscle cell, which appeared compressed typically. 90% cells in suspension were identified smooth muscle cells on EM. We conclude that the method for isolation of pulmonary arterial smooth muscle cells is simple, stable and effective and is recommanded for use.