镉在小鼠肢芽器官体外培养中的致畸性及锌对其致畸作用的影响

Application of Mouse Limb Bud Culture to Study the Influenceof Zinc on Teratogenesis Induced by Cadmium

采用体外小鼠胚胎肢芽器官培养方法，在培养基中加入不同浓度的硫酸镉及在加镉的同时加入不同浓度的锌，观察镉对肢体发育和分化的作用及锌对镉作用的影响。结果显示：随着镉剂量的增加，前后肢体软骨原基的发育和分化越差，软骨面积越小。０．１μｇ／ｍｌ剂量可导致指（趾）骨畸形，镉对软骨的作用比对软组织明显，长骨和爪骨均受到明显的影响；而加入镉后，随着加锌量的增加，大多数软骨原基的发育分化程度得到改善，软骨面积增大，长骨增长比爪骨明显，说明锌在一定程度上能够拮抗镉对软骨的抑制作用。

In this study, an in vitro method of mouse limb bud culture in selfmade rotator with continuous supplementation of gas mixture was employed in studying the teratogenic potential of cadmium and the influence of zinc on the teratogenesis induced by cadmium. Image analysis on the area and the form of the bone analgen of the cultured limb was used to evaluate quantitatively their teratogenic potentials.Different amounts of cadmium were directly added to culture medium. As cadmium concentrations were increased from 0.1 to 1.0 μg/ml, the degree of morphogenetic differentiation and the area of the bone anlagen of limbs culture were significantly decreased. The paws and long bones were affected seriously. Cadmium had a greater effect on chondrogenic tissue than on soft tissue. Then various levels of Zn, together with cadmium (1.0μg/ml medium), were added into the culture media. As Zn concentrations increased from 1.0 to 10.0μg/ml, the degree of morphogenetic differentiation and the area of cartilaginous bone anlagen of limbs culture were improved or increased. The long bones were better ameliorated as compared with the paw.