东北红豆杉内生真菌代谢产物的化学研究

Chemical studies on metabolites of an endophyticfungus associated with Taxus cuspidata

在寻找微生物活性代谢产物的过程中，发现东北红豆杉内生真菌ＴＣＨ６８的发酵液能够使细胞有丝分裂停止在中期，从其培养物中分离得到２个新化合物ＴＣＨ６８－１和ＴＣＨ６８－２。经光谱分析确定ＴＣＨ６８－１的结构为２，５－ｄｉｏｘｏ－３－ｍｅｔｈｙｌ－８，９，１０－ｔｒｉｍｅｔｈｏｘｙ－９－ｃｈｌｏｒｏ－１，１０６，８－ｄｉｌａｃｔｏｎｅ。ＴＣＨ６８－２的结构为２－［（Ｚ）－１－ｏｘｏ－２，３－ｄｉｈｙｄｒｏｘｙ－４－ｈｅｐｔｅｎｙｌ］－２－ｅｎｅ－３－ｍｅｔｈｙｌ－４－ｏｘｏ－５－ｈｙｄｒｏｘｙ－６－ｍｅｔｈｏｘｙ－６－ｂｅｎｚｏｙｌ－ε－ｃａｐｒｏｌａｃｔａｍ。

During the course of searching for microbial active metabolites, the fermentation broth of hyphomycete TCH68, an endophytic fungus associated with the inner bark of Taxus cuspidata Sieb. et Zucc., was found to induce mitotic arrest in vitro. Two novel compounds, TCH681 and TCH682 have been isolated from the culture by silica gel column chromatography and preparative TLC. Based on spectral data, including UV, IR, FAB-MS, HREI-MS, ~1 HNMR, ^(13)CNMR, DEPT, 1H1H COSY, HETCOR and HMBC, the structures of TCH681 and TCH682 have been assigned as 2, 5-dioxo-3-methyl-8, 9, 10-trimethoxy-9-chloro-1, 10∶6, 8-dilactone and 2-[(Z)-1-oxo-2, 3-dihydroxy-4-heptenyl]-2-ene-3-methyl-4-oxo-5-hydroxy-6-methoxy-6-benzoylεcaprolactam respecitively.