微生物来源的胆固醇生物合成酶抑制剂Ⅶ.抗生素SIPI┐8917┐Ⅰ的研究

Cholesterol biosynthetic enzyme inhibitor from microorganismsⅦ.Study on antibiotic SIPI8917Ⅰ

应用胆固醇生物合成的体外酶反应筛选系统，从１０００多株真菌中筛选出具有抑制胆固醇生物合成活性的ＳＩＰＩ－８９１７菌株；对ＳＩＰＩ－８９１７菌株的代谢产物进一步研究，应用丙酮浸泡、溶媒萃取、硅胶柱分离和ＬＨ２０凝胶层析等方法，从其菌丝体中分离出ＳＩＰＩ－８９１７－Ⅰ化合物。通过元素分析和质谱，确定其分子式为：Ｃ２２Ｈ２０Ｏ７（分子量３９６．２９０５），综合ＵＶ、ＩＲ、１Ｈ－ＮＭＲ、１３Ｃ－ＮＭＲ及ＨＭＢＣ和ＨＭＱＣ等图谱数据的解析，确定其结构为蒽环类化合物，与文献报道的１，３－二甲醚奥佛尼红素（１，３－Ｄｉ－Ｏ－ｍｅｔｈｙｌａｖｅｒｕｆｉｎ）结构一致。

Using a method for screening of cholesterol biosynthetic enzyme inhinitors from microorganisms in an enzyme system in vitro, a positive strain named SIPI8917 was obtained from more than 1000 fungi collected in our laboratory, whose metabolites exhibited inhibitory activities on cholesterol biosynthetic enzyme.Physiologically active compound SIPI8917Ⅰ was isolated from the mycelia of fungi SIPI8917 by acetone soaking, solvent extraction, silica gel column, LH20 column chromatography etc. Based on EIMS, FAB and HRMS, its formula was determined to be C_22H_20O_7 (MW 396.2905). By analyses of UV, IR, 1HNMR, 13 CNMR, HMQC and HMBC etc. spectra data, the structure of SIPI8917Ⅰ was elucidated and was identical with that of 1, 3-di-O-methylaverufin.