益肾通脉汤对兔动脉粥样硬化平滑肌细胞凋亡及Bcl-2 Caspase-3的影响

Effects of Yishen Tongluo Decoction on Apoptosis of Smooth Muscle Cell Bcl-2 and Caspase-3 in Atherosclerosis of Rabbits

目的:探讨动脉粥样硬化平滑肌细胞(smooth muscle cell,SMC)凋亡的机制及益肾通脉汤对其的干预作用。方法:高脂饲料喂养及耳缘静脉注射牛血清白蛋白复制兔动脉粥样硬化(atherosclerosis,AS)模型,同时造模开始即以益肾通脉中药进行早期干预,12周后,取胸主动脉,TUNEL染色检测SMC凋亡率及SP法检测Caspase-3和Bcl-2的阳性表达率。结果:益肾通脉组与模型组比较,斑块中SMC凋亡数量明显减少(P<0.05),益肾通脉组Bcl-2阳性表达率明显低于模型组(P<0.05),益肾通脉组Caspase-3阳性表达率明显低于模型组(P<0.05)。结论:益肾通脉汤可以抑制兔AS斑块中SMC的凋亡,从而延缓或者阻碍了AS的发展,其作用机制可能是通过上调Bcl-2表达而抑制Caspase-3表达的这个途径。

Objective ： To investigate the atherosclerosis can be alleviated by the assistance of SMC （ smooth muscle cell, SMC） apoptosis through the Yishen Tongluo decoction intervening to atherosclerosis model in rabbits. Methods： To make atherosclerosis model with BSA （ bull serum albumin, BSA） injection through the marginal vein of the ear and feed them high lipid diet. Make the observation of the pathology and the SMC apoptosis. Results ： The inner and medial membrane of the Yishen Tongluo group and the model group are thicker compared with the control group. The inner and medial membrane of the Yishen Tongluo group is thinner than the model group. There is little apoptosis in the control group and the apoptosis in Yishen Tongluo group increased significantly compared with the model group. Conclusion： It is possible that the Yishen Tongluo decoction to alleviate and retard the development of the atherosclerosis plaque.