乙型肝炎病毒前C区1896位点突变的PCR分析法

Analysis of precore region 1896 site mutation of hepatitis B virus by polymerase chain reaction

目的分析乙型肝炎病毒（ＨＢＶ）前Ｃ区突变与ＨＢＶ感染患者肝脏病变的关系，建立ＨＢＶ前Ｃ区１８９６位点突变的ＰＣＲ分析方法。方法根据ＨＢＶ前Ｃ区ＤＮＡ序列，采用３′－碱基特异性聚合酶链反应分析ＨＢＶ第１８９６位核苷酸的突变，并检测了１２６例临床血清标本。结果成功分析了ＨＢＶ前Ｃ区１８９６位点突变，１２６例临床标本突变株检出率为４７．２％。结论本法操作简单，重复性好。

Objective The polymerase chain reaction(PCR) analysis of precore region 1896 site mutation of hepatits B virus(HBV) was applied in order to study relationship between the mutation of HBV precore and liver damage. Methods According to the DNA sequence of HBV precore, the mutation of HBV precore 1896 site has been detected in 126 serum samples by simple method3′BSPCR.Results The HBV precore 3′BSPCR has been established and mutation rate was 47.2%. Conclusions The HBV precore 3′BSPCR can be as a new method for studying HBV infection and analysing liver damage.