自然杀伤细胞的独立刺激信号——CD45分子交联

CD45 crosslinking is the independent stimulatory signal in natural killer cells activation

目的：探讨表面分子在自然杀伤（ＮＫ）细胞活化中的信号分子作用及其机制。方法：采用多种粘附分子单抗及配体刺激ＮＫ细胞，ＥＬＩＳＡ法定量检测刺激后ＮＫ细胞释放的γ干扰素（ＩＦＮ－γ）作为ＮＫ细胞活化指标，观察各种粘附分子在ＮＫ细胞释放ＩＦＮ－γ中的信号作用。结果：单一ＣＤ４５单抗刺激即可使ＮＫ细胞活化；两种分别为ＩｇＧ１，ＩｇＧ２ａ亚类的ＣＤ４５单抗及ＩｇＧ２ａ亚类ＣＤ４５单抗的Ｆ（ａｂ）２均体现对ＮＫ细胞的独立刺激作用。结论：ＣＤ４５分子介导的ＮＫ细胞活化与ＣＤ１６－Ｆｃ段间相互作用无关。

Objective: To determine the identity of molecules and mechanism that mediate signal transduction and NK cell activation. Methods: We quantitated IFNγ concentration produced by NK cells that were stimulated with a panel of purified ligands and monoclonal antibodies specific for adhesion molecules. Results: AntiCD45 mAbs could specifically and independently stimulated IFNγ production in NK cells. The ability of CD45 mAbs to stimulate NK cells appears not to be dependent on CD16Fc partition interaction, as CD45 mAbs of both IgGl and IgG2a isotypes,both whole antibodies and its F(ab)2,were equally stimulatory. Conclusion: These observations suggest that CD45 crosslinking is the independent and unique stimuli for NK cell activation.