适合RT-PCR的苹果芽RNA快速提取方法研究

Study on a Rapid Extraction Method of RNA from Apple Bud for RT-PCR

[目的]从苹果单芽中高效、快速提取RNA,为后续分子生物学研究奠定基础。[方法]分别以苹果品种新红星、嘎拉和富士的单芽为材料,用改良SDS法提取RNA,并经过甲醛变性琼脂糖凝胶电泳和RT-PCR检测RNA质量。[结果]研究出一种高效、快速的苹果单芽RNA提取方法,该方法能在2.5 h内得到完整的RNA,经RT-PCR检验,能获得理想的目的片段。[结论]该方法稳定可靠,适合苹果单芽RNA提取。

[ Objective ] The study aimed to establish an effective and rapid protocol for RNA extraction from single apple bud, which was benificial to subsequent molecular biological research. [ Method] RNA from apple cultivars ‘ Starkrimson＇ , ‘ Gala＇ and ‘ Fuji＇ buds was extracted by modified-SDS method, and RNA was detected by formaldehyde denaturing agarose gel electrophoresis and RT-PCR. [ Result] An effective and rapid RNA extraction protocol was established, which could be completed in 2.5 h. RNA extracted through this protocol was detected by RT-PCR and an ideal target fragment was obtained. [ Conclusion ] The method is stable and reliable, and suitable for RNA extraction from single apple bud.