鹅副粘病毒GX1株的HN和F蛋白基因的克隆和序列分析

Cloning and Sequence Analysis of HN and F Proteins Encoding Genes from a Strain of Goose Paramyxovirus

[目的]克隆鹅副粘病毒GX1株HN基因与F基因，并进行序列分析。[方法]根据Genbank已发表的鹅副粘病毒GPV—SF02株基因组核苷酸序列设计2对引物，对从广西发病鹅分离到的鹅副粘病毒毒GX1株的HN和F基因进行PCR扩增，将扩增产物与pMD18-T载体连接并测序。[结果]该株副粘病毒株的HN基因和F基因核苷酸序列全长分别为1716和1662bp，与GPV—SFO2株的同源性均在97．3％左右，与LaSota株、F48E9株、JS株的同源性为80．3％～97．5％，与Miyadera株的同源性仅84．8％。[结论]分离株GX1株与禽Ⅰ型副粘病毒（APMV-1）强毒株特征相符，属于APMV-1基因Ⅶ型。

[ Objective ] The study was to clone HN and F genes from GX1 strain of goose paramyxovirus and analyze their sequences. [ Method] According to the full nucleotide sequence of GPV-SFO2 strain of goose paramyxovirus, two pairs of primers were designed to amplify the HN and F genes from GX1 strain of goose paramyxovirus isolated in diseased goose in Guangxi Zhuang Autonomous Region; the amplified products were ligated into pMD18-T vector and sequenced. [ Result] HN and F genes of this strain tested were 1 716 and 1 662 bp in full nucleotide length, respectively; both showed the homologues of about 97.3% with GPV-SFO2 strain, of 80.3% -97.5% with strains LaSota, F48E9 and JS, of just 84.8% with Miyadera strain. [ Conclusion] The results show that isolated strain BX1 matches tovirulent APMV-1 strain, belonging to genotype Ⅶ of APMV-1 strain.