HPLC法测定不同产地贯叶连翘中黄酮类成分的含量

Determination on Flavone Ingredients in Hypericum perforatum L.from Different Producing Areas by HPLC Method

采用HPLC法测定不同产地贯叶连翘中芦丁、金丝桃苷和槲皮素的含量。结果表明，芦丁的线性检测范围为0．0529—0．5290μg（r=0．9997），平均回收率为99．4％，RSD=1．471％；金丝桃苷的线性检测范围为0．0493～0．4930μg（r=0．9996），平均回收率为100．2％，RSD=1．407％；槲皮素的线性检测范围为0．00166～0．05300μg（r=0．9998），平均回收率为98．5％，RSD=1．295％。

The contents of rutin, hyperin and quercetin in Hypericum petforatum L. from different producing areas were determined by HPLC method. The results showed that the linear detection range of rutin was 0.052 9 -0. 529 0 μg （ r = 0. 999 7 ） with average recovery of 99.4% and RSD of 1.471%. The linear detection range of hyperin was 0.049 3 -0.493 0 μg （r = 0.999 6 ） with average recovery of 100.2% and RSD of 1. 407%. The linear detection range of quereetin was 0. 001 66 -0.053 00 μg （ r = 0.999 8 ） with average recovery of 98.5% and RSD of 1. 295%.