小桐子胚愈伤组织耐盐性研究

Study on Salt Tolerance of Embryo Callus of Jatrohpa curcas

[目的]筛选耐盐性较强的小桐子新品种。[方法]以小桐子种胚为材料,采用含不同浓度6-苄基腺嘌呤(6-BA)和吲哚丁酸(IBA)组合的培养基对其进行愈伤组织诱导,筛选出最佳诱导培养基;在最佳诱导培养基中分别加入0、20、40、60、80、100 mmol/L NaCl对小桐子胚愈伤组织进行诱导,研究胚愈伤组织的耐盐性。[结果]小桐子胚愈伤组织的最佳诱导培养基为MS+1.0 mg/L6-BA+0.1mg/LIBA;胚愈伤组织在含40、60 mmol/LNaCl的培养基上生长状况最佳,当培养基中NaCl浓度达到100 mmol/L时,小桐子胚愈伤组织无法存活。[结论]在MS+1.0 mg/L6-BA+0.1 mg/LIBA培养基中,小桐子胚愈伤组织具有较好的耐NaCl性。

[ Objective ] The aim was to select new Jatrohpa curcas variety with strong salt tolerance. [ Method ] With seed embryos of J. curcas as the materials, the embryo callus was induced with the mediums that contained different conch, of 6 benzyl adenine （6-BA） and indole butyric acid （IBA）, and then the optimum induction medium was screened out. And then 0, 20, 40, 60, 80, 100 mmol/L NaCl was added into the optimum induction medium resp. to induce embryo callus of J. curcas, and the salt tolerance of the embryo callus was studied. [ Result ] The optimum induction medium for embryo callus of J. curcas was MS ＋ 1.0 mg/L 6-BA ＋ 0. 1 mg/L IBA. The growth status of embryo callus in mediums that contained 40, 60 mmol/L NaCl were the best. When NaCl in medium reached 100 mmol/L, the embryo callus of J. curcas could not survival. [ Conclusion] The embryo callus of J. cureas in medium of MS ＋ 1.0 mg/L 6-BA ＋0. l mg/L IBA had better salt tolerance.