儿童腹泻轮状病毒G和P基因分型特征分析

Molecular characteristic of G-type and P-type group A rotavirus among children with diarrhea.

目的调查浙江温岭市儿童腹泻A组轮状病毒的感染情况和基因分型特点。方法本院2008年1月至2008年12月收集的腹泻儿童1 560份粪便标本采用酶联免疫吸附试验和逆转录-巢式聚合酶链反应,进行轮状病毒病原检测,并对阳性标本进行VP7和VP4基因分型。结果本市1 560例儿童腹泻有309份标本为A组轮状病毒阳性(阳性率为19.8%)。G基因型以G3型为主(49.8%),其次为G1(24.6%)及混合G型(13.3%);P基因型以P[8]型为主(55.3%),其次为P[4](23.3%)。结论A组轮状病毒是儿童腹泻的重要病原体,其中G3P[8]型为主要基因型。

Objective： To investigate the epidemiology and genotypes of Group A Rotavirus among children with diarrhea. Methods ： From January to December 2008, 1 560 stool specimens collected from children with diarrhea, were tested by enzyme - linked immunosorbant assay （ELISA） and reverse transcription nested polymerse chain reaction （ RT - nPCR） to identify the rotavirus infection and the G/P genotypes respectively. Results ： Enzyme - linked immunosorbant assay （ELISA） results showed that Rotavirus A was present in 19. 8% （309 cases） of the 1560 specimens without gender priority. G genotype identified G3 at 49. 8%, G1 24.6% and mixed - G 13.3%. P genotype identified P [8] at 55.3% and P [4] 23.3%. Conclusion： Rotavirus A was the significant pathological agent of children with diarrhea. G3 and P [ 8 ] were the most prevalant G/P genotypes.