妊娠早期绒毛及中晚期胎盘中Ghrelin的表达研究

Expression of the Ghrelin in early pregnancy villi and late in the placenta.

目的探讨明确ghrelin是否参与了妊娠胎盘形成发育及胚胎早期发育进程。方法本研究利用免疫组化和实时定量RT-PCR技术检测了人妊娠早期绒毛及中晚期胎盘ghrelin蛋白的表达定位和ghrelin mRNA水平相对表达变化规律。结果免疫组化结果表明,ghrelin蛋白主要分布于滋养层细胞和血管内皮细胞;实时定量RT-PCR结果揭示人妊娠早期绒毛及中晚期胎盘ghrelin mRNA的相对表达量依据发育阶段的不同而呈现一定变化规律,即在妊娠早期6、7、8周显著高于12-15周胎盘(P<0.05),16周表达量又出现上升趋势。在晚期胚胎中ghrelin表达明显下降。结论妊娠早期绒毛和中晚期胎盘中ghrelin蛋白的表达及ghrelin mRNA特定的表达模式,揭示这一新型分子在人妊娠早期绒毛形成及中晚期胎盘发育过程中具有潜在的调控作用。

Objective： The possibility of ghrelin expressed in early pregnancy villi and late in the placenta was investigated in this study. Methods ： The study observed ghrelin protein expression positioning and mRNA level of expression of the relative changes in the law in early pregnancy villi and late in the placenta by immunohistochemical and real - time quantitative RT - PCR technology. Results： Immunohistochemistry results showed that ghrelin protein mainly located in the trophoblast ceils and endothelial ceils. Real - time quantitative RT - PCR revealed the results of human chorionic villi and early pregnancy in the late placenta ghrelin mRNA expression of the relative amount of development based on the different stages of a certain change in the law, That is, 6, 7, 8 in the early weeks of pregnancy were significantly higher than the placenta 12 - 15 weeks （ P 〈 0.05 ）, 16 weeks expression appeared on the rise. Conclusion ： Late in the embryos decreased expression of ghrelin. Villi in early pregnancy and late in the placental protein expression of ghrelin and ghrelin mRNA expression of a specific model, to reveal the new elements in the human form a pile of early pregnancy and late in the process of development of the placenta has the potential role of regulation and control.