小鼠原位肝移植模型建立方法的改良研究

Improved method of orthotopic liver transplantation model in mice

目的探讨建立小鼠原位肝移植模型的技术改进方法。方法参照"Qian SG"法建立小鼠原位肝移植模型,对供肝的灌注、切取、修整及受体肝上下腔静脉吻合等方法进行改良,分别用传统方法(对照组)和改进后的方法(实验组)各完成小鼠原位肝移植70例,比较两组间供、受体手术时间及48h、1周、1个月的存活率。结果实验组供体手术时间、受体手术时间、无肝期时间分别为37±2min、45±2min、16±2min,均较对照组(分别为45±2min、54±2min、23±2min)明显缩短(P<0.05)。实验组与对照组48h存活率(分别为95.7%、91.4%)比较无显著性差异(P>0.05),但实验组1周、1个月存活率(分别为92.9%、85.7%)较对照组(分别为81.4%、71.4%)均明显提高(P<0.05)。结论该方法能有效地缩短供、受体手术时间,提高供肝质量及受体长期存活率,是一种稳定、可靠、有效的小鼠肝移植建模方法。

Objective To improve the method of orthotopic liver transplantation model in mice. Methods On the basis of ＂Qian SG＂ mothod, the techniques of perfusion, harvesting, and trimming of donor＇s liver, as well as the anastomosis of superior and inferior caval veins were improved. Operations were performed in 70 mice with improved technique （experiment group） and in 70 mice with conventional technique （control group）, respectively. The operation time in both donors and recipients, and the survival rates of 48h, 1 week and 1 month after operation were compared between the two groups. Results The operation time for both donors and recipients and the time of anhepatic phase were 37±2min, 45±2min and 16±2min, respectively in experiment group, while they were 45±2min, 54± 2min and 23±2min, respectively, in control group, so that the average time for each procedure was significantly shorter （P〈0. 05） in experiment group than in control group. The survival rates of 1week and 1--month after operation in experiment group were 92. 9% and 85. 7% , respectively, and they were significantly higher than that in control group （81.4% and 71.4%）, indicating that the improved techniques raised the survival rates of 1 week and 1 month after operation remarkably （P〈0. 05）, but the survival rate of 48 hours after operation showed no statistical significance between experiment group （95. 7% ） and control group （91.4%, P〉0. 05）. Conclusions The improved technique may shorten the operation time and raise the survival rate, and is an ideal method for the establishment of orthotopic liver transplantation model in mice.