北京市男男性接触HIV-1感染者膜蛋白基因序列测定及亚型分析

Env gene sequencing and HIV-1 subtyping of infected MSM from Beijing

目的了解北京市男男性接触人群（MSM）中HIV-1的最新流行趋势及膜蛋白v3环序列特征。方法巢式聚合酶链式反应（n．PCR）扩增2007年提取的北京市男男性接触HIV感染者基因组DNA样品，对膜蛋白基因c2-v3区测序，进行病毒亚型及v3环序列特点分析。结果11例样本中，4例是欧美B亚型，5例是AE重组亚型，1例是Bc重组亚型，1例是01B重组亚型。v3环顶端四肽以GPGQ和GPGR为主。结论北京市男男性接触HIV-1感染者中重组亚型呈蔓延流行趋势。

Objective To comprehend the latest HIV- 1 epidemic tendency and the character of V3 loop in MSM population of Beijing. Methods The C2-V3 regions of the HIV envelop gene were amplified by nest-PCR and sequenced from 11 HIV-1-infected MSM in Beljing in 2007. The subtype and sequences of V3 loop was analyzed. Results There are 4 subtype B strains, 5 CRF AE, 1 CRFO7BC and 1 CRF15-01B strains within all 11 strains. There are five types of central motifs of the 11 samples, in which GPGR and GPGQ are most common. Conclusion Recombination subtype of HIV-1 are spread extensively in MSM population of Beijing.