α-干扰素中和抗体对慢性乙肝患者干扰素抗病毒疗效的影响

Role of neutralizing anti-interferon antibodies during interferon therapy in patients with chronic hepatitis B

目的研究慢性乙型肝炎患者α-干扰素（IFN—α）中和抗体（NA）产生的情况，并探讨其对IFN抗病毒疗效的影响。方法采用抗病毒中和生物测定法检测了48例慢性乙型肝炎患者IFN-α治疗前、治疗后3—6个月血清中NA产生的情况；同时，也检测了10名健康人血清中NA。结果慢性乙型肝炎患者IFN—α治疗前和健康人血清中均无NA产生。48例患者IFN—α治疗6个月后，完全应答有15例，部分应答23例，无应答10例；IFN—α治疗后3个月和6个月时血清中NA阳转率分别为25％和37．5％（P〉0．05）。治疗后3个月时完全应答组和部分应答组NA阳转率均显著高于无应答组；同时，治疗后6个月时完全应答组NA阳转率较无应答组也显著升高。结论IFN—α治疗后机体可产生NA；NA的产生可影响IFN—α抗病毒疗效，尤其是IFN—α治疗后早期（3个月）即产生了NA。

Objective Investigation in the role of neutralizing anti-interferon antibodies （NA）during interferon therapy in patients with chronic hepatitis B. Methods To measure the levels of NA in 48 patients with chronic hepatitis B（CHB） treated with interferon-α（IFN-α） at the point before treated and at 3 and 6 months with treatment respectively. At the same time to measure the levels of NA in health volunteers. Results NA can not be measured in health volunteers and those patients with CHB at the point before treated. At the point of 6 months with treatment, 15 patients with CHB got complete respond, 10 patients with CHB got no respond, 23 patients with CHB got partly respond, respectively. NA positive rate was 25% at 3 months and 37.5 % at 6 months with treatment, respectively. （P 〉 0.05）. NA positive rate was significantly higher in complete responders group and partly responders group than in no responders group at 3 months with. NA positive rate was significantly higher in complete responders group than in no responders group at 3 months with. Conclusion NA can be measured in blood serum after treated with interferon. NA influences the efficacy of interferon, especially at 3 months.