Survivin、COX-2及VEGF在大肠癌中的表达及与肿瘤微血管密度的关系

Expression of Survivin,COX-2 and VEGF in colorectal cancer and its correlation with tumor angiogenesis

目的:研究Survivin,COX-2,VEGF和肿瘤微血管密度(MVD)在大肠癌组织中的表达,探讨其与大肠癌肿瘤血管生成的关系.方法:2007-09/2008-05哈尔滨医科大学附属第二临床医学院内镜下取材的大肠癌、肠息肉及肠炎标本.所有标本均经病理检查证实诊断.试验对象共分3组,分别为大肠癌组织26例,大肠息肉组织10例,大肠黏膜慢性炎症组织7例.采用免疫组织化学方法检测Survivin,COX-2,VEGF和CD34在大肠组织中的表达.结果:Survivin,COX-2与VEGF蛋白在大肠癌组织中阳性表达率分别为76.9%,80.8%和69.28%,明显高于大肠息肉组与肠炎组的表达(P<0.01或0.05).大肠癌组织中MVD(CD34)明显高于大肠息肉组与肠炎组(23.69±9.96vs13.10±7.05,10.43±4.24,均P<0.01).Survivin,COX-2和VEGF蛋白在大肠癌组中的表达与MVD相关(均P<0.05),Survivin和促血管形成因子COX-2,VEGF在大肠癌组织中的表达密切相关(χ2=11.18,4.72,均P<0.005).结论:Survivin可能通过COX-2,VEGF促进大肠癌肿瘤血管的形成.

AIM： To simultaneously detect the expression of Survivin, cyclooxygenasa-2 （COX-2）, vascular endothelial growth factor （VEGF） and CD34 in colorectal cancer and explore its correlation with tumor angiogenesis. METHODS： All specimens （including 26 colorec- tal cancer specimens, 10 colorectal polyp speci- mens and 7 chronic colitis specimens） were collected from September 2007 to May 2008 at the Second Affiliated Hospital of Harbin Medical University. All specimens were pathologically reevaluated to confirm the initial diagnosis. The expression of Survivin, COX-2, VEGF and CD34 in these specimens was detected by immunohis- tochemistry. RESULTS： The positive rates of Survivin, COX-2 and VEGF proteins in colorectal cancer tissue were 76.9%, 80.8% and 69.28% respectively, significantly higher than those in colorectal polyp and chronic colitis tissues （P 〈 0.01 or 0.05）. The microvascular density （MVD） revealed by CD-34 immunostaining in colorectal cancer tissue was significantly higher than that in colorectal polyp and chronic colitis tissues （23.69 ± 9.96 vs 13.10 ± 7.05 and 10.43 ±4.24, both P 〈 0.01）. Furthermore, the expression of Survivin, COX-2 and VEGF proteins in colorectal cancer tissue is positively correlated with tumor microvessel count （all P 〈 0.05）. In colorectal cancer tissue, the expression of Survivin was closely correlated with that of COX-2 and VEGF （χ^2=11.18, 4.72, P 〈 0.05）. CONCLUSION： Survivin is probably abIe to promote tumor angiogenesis through regulating the expression of COX-2 and VEGF in colorectal cancer