电针足三里对脓毒症大鼠小肠促炎症因子、二胺氧化酶活性及组织含水率的影响

Effects of electro-acupuncture at Zusanli point on the expression of proinflammatory cytokines,the activity of diamine oxidase and the rate of water content in the small intestine in rats with sepsis

目的:探讨电针足三里(ST36)对脓毒症大鼠促炎症因子所致小肠损伤的抑制作用.方法:采用盲肠结扎穿孔术(CLP)制备大鼠脓毒症模型,取♂SD大鼠32只,随机分为CLP+电针(EA)足三里组(CLP+EA组)、CLP+假电针(shameEA)(CLP+SEA)组、迷走神经切断(VA)+CLP(VA+CLP)组和迷切后CLP再电针(VA+CLP+EA组)组,每组8只.EA组持续针刺双侧足三里30min,强度为2mA,2-100Hz.CLP/SEA组采用相同频率和强度刺激非经非穴(足三里外侧旁开0.5cm)30min.VA组先手术切断腹腔迷走神经再CLP.各组大鼠于CLP术后6h处死取空肠组织,检测TNF-α和NO含量、髓过氧化物(MPO)和二胺氧化酶活性及肠组织含水率.结果:采用盲肠结扎穿孔术后6h,与其余3组相比,CLP/EA组小肠组织促炎因子TNF-α、NO、MPO水平和组织含水率均显著降低、DAO活性显著升高(均P<0.05).VA+CLP组和VA+CLP+EA组TNF-α、NO和MPO水平显著高于CLP+SEA组(748.80±112.45pg/gprotein,737.58±100.56pg/gproteinvs560.23±82.25pg/gprotein;1.32±0.15μmol/gprotein,1.12±0.24μmol/gproteinvs0.97±0.12μmol/gprotein;0.57±0.06U/g,0.61±0.12U/gvs0.45±0.07U/g,均P<0.05),DAO活性显著低于CLP+SEA组(0.07±0.02U/L,0.06±0.04U/Lvs0.12±0.04U/L,均P<0.05);VA+CLP+EA组、VA+CLP组、CLP+SEA组上述指标的变化无统计学差别.结论:电针足三里显著抑制CLP大鼠小肠促炎症因子水平,减轻肠组织水肿和功能损害.

AIM： To investigate the inhibitory ettects of electro-acupuncturing （EA） at Zusanli （ST36） against proinflammatory cytokine-induced intestinal injury in rats with sepsis. METHODS： Thirty-two male Sprague-Dawley rats were subjected to cecal ligation and puncture （CLP） to develop a rat model of sepsis. The rats were then randomly divided into four groups （n = 8 each）： CLP plus EA group, CLP plus sham EA （SEA） group, vagotomy （VA） plus CLP group and VA plus CLP plus EA group. An electroacupuncture at Zusanli point （ST36） was performed at constant voltage （2-100 Hz, 2 mA for 1.5 h） immediately after CLP surgery. Rats in the VA plus CLP group and the VA plus LPS plus EA group were abdominally vagotomized. All animals were sacrificed at 6 h after CLP to collect jejunal specimens. The contents of tumor necrosis factor-or （TNF-α） and nitric oxide （NO）, the activity of myeloperoxidase （MPO） and diamine oxidase （DAO）, as well as the rate of water content （ratio of dry weight to wet weight） were determined. RESULTS： The levels of TNF-α and NO, the activity of MPO and the rates of water content in the jejunum in the CLP plus EA group were significantly lower than those in the other three groups, while the activity of DAO at 6 h after CLP in the CLP plus EA group was significantly higher than that in the other three groups （all P 〈 0.05）. The levels of TNF-α and NO and the activity of MPO in the VA plus CLP group and the VA plus CLP plus EA groups were significantly higher （748.80 ± 112.45 pg/g protein and 737.58 ± 100.56 pg/g protein vs 560.23 ±82.25 pg/g protein; 1.32 ± 0.15 μmol/g protein and 1.12 ± 0.24 μmol/g protein vs 0.97 ± 0.12 μmol/g protein; 0.57 ± 0.06 U/g and 0.61±0.12 U/g vs 0.45 ± 0.07 U/g, all P 〈 0.05） than those in the CLP plus SEA group, while the activity of DAO in the VA plus CLP group and the VA plus CLP plus EA groups was significantly lower than that in the CLP plus SEA group （0.07 ± 0.02 U/L and 0.06 ± 0.04 U/L vs 0.12± 0.04 U/L, both P 〈 0.05）. There were no significant differences in all of the above parameters between the VA plus CLP plus EA group and the VA plus CLP group. CONCLUSION： Electro-acupuncture at Zusanli point significantly lowers the elevated levels of proinflammatory factors in the small intestine and alleviate tissue edema and mucosal dysfunction in rats undergoing CLP.