Survivin及VEGF在脉络膜黑色素瘤的表达及其与血管形成的关系

Relationship of survivin and VEGF with angiogenesis in choroidal melanoma

目的研究脉络膜黑色素瘤(CM)组织中survivin、血管内皮生长因子(VEGF)与微血管密度(MVD)的相关性,探讨survivin的作用机制。方法采用免疫组织化学SP法检测survivin、VEGF在58例CM组织和8例正常脉络膜组织中的表达,利用CD105标记CM组织的新生血管。结果58例CM组织中survivin蛋白表达阳性率为69%,VEGF阳性率为74%,二者在正常脉络膜组织中均不表达。Survivin和VEGF与患者在不同年龄、肿瘤大小、最大基底径、肿瘤生长方式、巩膜壁浸润与否间差异无统计学意义(P>0.05),但与CM的巩膜导管侵犯与否间的比较差异有统计学意义(P<0.05);survivin、VEGF在CM组织中的表达与否与MVD的比较差异有统计学意义(P<0.05);survivin和VEGF在CM组织中的表达密切相关(r=0.522,P<0.01)。结论Survivin和VEGF在血管生成过程中密切相关,survivin蛋白可作为CM靶向治疗的靶点。

Objective Survivin is a vascular sheltered gene and it is associated with tumor neovascularization. Present study aimed to investigate the correlation of survivin and vascular endothelial growth factor （VEGF） with microvessel density （MVD） in choroidal melanoma and explore the mechanism of survivin as a anti-tumorigenesis factor. Methods Fifty-eight specimens of choroidal melanoma tissue were collected,and 8 specimens of normal choroid tissue were obtained from normal eyes of donors in eye bank of Beijing Tongren Hospital. The expression of survivin and VEGF in collected specimens was detected by using immunohistochemistry. New blood vessel in choroidal melanoma tissue was marked with CD105 using immunohistochemistry. Results The expression of survivin and VEGF in choroidal melanoma was 69% and 74%, respectively. No expression of survivin and VEGF was detected in normal choroid tissue. No significant differences were found between survivin or VEGF and age, tumor size, maxium tumor diameter, sclera infiltration of tumor（ P 〉 0.05 ）. Expression rates of survivin and VEGF were significantly enhanced in the specimen with invasion of scleral emissaria in comparison with specimen without invasion of scleral emissaria（P 〈 0. 05 ）. MVD was considerably increased in positive expressed specimen of survivin or VEGF compared with absent expression（ P 〈 0.05 ）. The expression of survivin was closely correlated to expression of VEGF（ r = 0. 522,P 〈 0. 01 ）. Conclusion Survivin and VEGF have intimate relation in angiogenesis. Survivin provide a new target therapy for choroidal melanoma.