六月青总皂苷对HepG 2.2.15细胞分泌HBsAg、HBeAg的影响

Effect of the terpenoids of Liuyueqing on HBsAg and HBeAg in the HepG 2.2.15 cells

目的:观察六月青总皂苷(TLYQ)的体外抗乙型肝炎病毒的作用。方法:采用HepG2.2.15细胞模型进行体外培养,给予不同浓度TLYQ,以拉米夫定作阳性对照,作用72h后检测上清液中HBsAg、HBeAg的分泌,观察药物对HepG2.2.15细胞分泌HBV病毒抗原的影响,同时以四甲基偶氮唑盐(MTT)比色法检测药物在体外对HepG2.2.15细胞的生长抑制作用,从而评价药物的抗HBV作用。结果:TLYQ对HepG2.2.15细胞的50%生长抑制率(TC50)为349.51mg·L-1,对HepG2.2.15细胞分泌HBsAg的50%抑制率(IC50)为9.45mg·L-1,其治疗指数(TI)为36.98。结论:TLYQ在体外有显著的抗HBV的作用,且毒性较低。

OBJECTIVE To observe the effect of the terpenoids of Liuyueqing （TLYQ） on excreting HBsAg and HBeAg of HepG2. 2. 15 cells. METHODS The toxicity of TLYQ on HepG 2. 2. 15 cells was detected by means of MTT. The inhibitory effects of TLYQ on HepG 2.2. 15 cells excreting HBsAg and HBeAg were evaluated with method of ELISA. RESULTS The half toxic concentration （TC50） of TLYQ on HepG 2. 2. 15 cells was 349. 51 mg.L^-1. The half inhibitory concentration （ICs0） of TLYQ on HBsAg was 9. 45 mg.L^-1 ,the trealment index （TI） of TLYQ on HBsAg was 36. 98. CONCLUSION TLYQ can inhibit significantly HBV in vitro, and is a lower toxic drug.