IGF-1在非梗阻性无精子症睾丸组织中的表达

Expression of IGF-1 in non-obstructive azoospermia testicle tissue

目的研究胰岛素样生长因子-1(IGF-1)在非梗阻性无精子症睾丸组织中的表达并探讨其意义。方法采用免疫组化EnVision法对32例非梗阻性无精子症人睾丸组织石蜡标本和13例人正常睾丸组织中的IGF- 1的表达进行检测,通过统计学方法分析其表达情况。结果非梗阻性无精子症睾丸组织HE染色显示生精小管中无或仅少量精子,生精小管的生精上皮脱落、排列紊乱,生精小管壁部分有玻变萎缩。32例非梗阻性无精子症睾丸组织中IGF-1阳性表达4例,阴性表达28例,阳性率12.5%;13例正常睾丸组织中IGF-1阳性表达10例,阴性表达3例,阳性率76.9%;差异有显著性(P<0.01)。结论(1)IGF-1在睾丸组织中表达减少或缺失与生精功能障碍密切相关。(2)非梗阻性无精子症睾丸组织生精小管的生精上皮脱落、排列紊乱,生精阻滞。

Objective To investigate the relationship between expression of IGF-1 in human testicle tissue and non-obstructive azoospermia. Methods The expression of IGF- 1 in testicle tissue of 32 non -obstructive azoospermia patients and 13 normal testicle tissue were detected by immunohistochemistry. The expression patterns of IGF-1 on the area and density of staining were comparatively analyzed using statistics methods. Results HE staining analysis showed naught or few amounts of zoosperm, seminiferous epithelium exuviation, arrangement disorder and spermatogenic cell atrophy in seminiferous tubules of the human non-obstructive azoospermia testicle tissue, The positive expression rate of IGF-1 in non-obstructive azoospermia testicle tissue was 12.5%, while in normal testicle tissue was 76.92%（P〈0.01）. Conclusion （1） Downregulated expression of IGF-1 in testicle tissue might be closely associated with spermatogenic dysfunction;（2）The pathological changes such as seminiferous epithelium exuviations, arrangement disorder and dyszoospermia were discovered in seminiferous tubules of the non-obstructive azoospermia testicle tissue.