藜叶中黄酮类化合物体外抗氧化活性研究

Study on Antioxidant Activity in vitro of Flavonoids from Chenopodium album L. Leaves

为了探讨藜叶中黄酮类化合物的体外抗氧化活性,采用有机溶剂提取法和色谱柱法对藜叶中化学成分进行提取与分离;以Vc作对照,对分离纯化的芦丁、乙酸乙酯浸膏和正丁醇浸膏进行DPPH·、O2-·和·OH的清除效果试验。结果表明:三者对DPPH·、O2-·、·OH均具有清除作用,且与浓度呈量效关系,芦丁对·OH清除效果优于Vc,芦丁具有较强的清除DPPH·能力,其IC50为0.05μg/mL。

In order to discuss antioxidant activity in vitro of flavonoids from Chenopodium album L. leaves, with solvent extraction method and chromatogram column method, rutin is acquired from Chenopodium album L. leaves; The free radical scavenging activities of rutin,ethyl acetate part and n-butanol part was studied in superoxide anion （O2^-）, hydroxyl radical（·OH）and DPPH· systems ,which is compared with Vc. The results showed the three samples have scavenging capability against DPPH · , O2^- and ·OH radleals in a dose dependent manner, rutin is stronger than Vc for scavenging ·OH radical,rutin has prominent effect of scavenging DPPH · radical,IC50 is 0.05 μg/mL.