非小细胞肺癌患者围术期血清MMP-9水平动态变化的临床研究

Clinical Research of Perioperative Serum MMP-9 Levels in Patients with Non-small Cell Lung Cancer

目的本研究通过监测非小细胞肺癌(non-small cell lung cancer,NSCLC)患者围术期血清基质金属蛋白酶-9(matrixmetalloproteinase-9,MMP-9)的水平,研究其动态变化规律。方法应用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测法,分别监测非小细胞肺癌及肺良性病患者术前、术后1、7、14、21及28d血清MMP-9的浓度,非小细胞肺癌患者21例,肺良性病患者12例,30例健康志愿者确定血清MMP-9正常水平。结果(1)NSCLC患者术前血清MMP-9浓度(82.84ng/mL)显著高于肺良性疾病患者术前(58.98ng/mL)及健康人血清水平(42.94ng/mL)(P=0.000);(2)手术切除肿瘤后,术后第1天非小细胞肺癌患者的血清MMP-9水平(265.87ng/mL)达峰值,比术前水平(82.84ng/mL)明显升高(P=0.000),此后下降。结论非小细胞肺癌患者血清MMP-9的术前水平显著高于肺良性病患者及健康人。围术期血清MMP-9水平均显著升高。

Objective Angiogenesis is necessary in tumor progression and metastasis.Serum matrix metalloproteinase-9（MMP-9） is important factor in angiogenesis. Elevated levels of MMP-9 is reported in lung cancer. This research was to study the perioperative dynamic changes of serum MMP-9 levels in non-small cell lung cancer（NSCLC） patients who undergo surgery. Methods Serum MMP- 9 levels were determined in 21 patients with NSCLC who were undergoing surgery by ELISA （enzyme linked immunosorbent assay） method before operation and on postoperative day 1, 7, 14, 21, 28. At the same time 12 patients with pulmonary benign diseases treated by surgery were studied as the control. Thirty healthy volunteers served to define normal MMP-9.Results （1） Preoperative serum MMP-9 level （82.84ng/mL） was significantly higher in the group of patients with NSCLC compared with the benign and healthy group （respectively： 58.98ng/mL and 42.94ng/mL,P=0.000）.（2）Serum MMP-9 levels were elevated soon after surgery and the peak was Observed on postoperative day 4-1 in NSCLC.Then serum MMP-9 levels dropped. Conclusion Preoperative serum MMP-9 level was significantly higher in the group of patients with NSCLC compared with the benign and healthy group. Periopecative serum MMP-9 levels were significantly elevated in patients with NSCLC.