RP-HPLC测定ZKT纳米粒中柠檬酸的含量

Determination of Citric Acid in ZKT Nanoparticles by RP-HPLC

目的：建立测定ZKT纳米粒中柠檬酸的方法。方法：采用RP-HPLC法，色谱柱为Dikma Platisil ODS—C18（250mm×4．60mm，5um），流动相为0．8％的NH4H2PO4（磷酸调pH2．8），流速为1．0ml·min^-1，柱温为30℃，检测波长为214nm。结果：柠檬酸进样量1．18—11．8ug与峰面积线性关系良好（r=0．9999），平均回收率为98．52％，RSD=0．85％（n=6）。结论：所用方法准确、快速、简便、重复性好，可控削ZKT纳米粒的质量。

Objective： To establish a method for determination of the citric acid in ZKT nanaoparticles. Methods： RP - HPLC was adopted.Citrlc acid was determined on a Dikma Platisil ODS C18 column （250mm × 4.6mm, 5um}, the mobile phase consisted of 0.8% ammonium dihydrogen phosphate solution （adjust pH to 2.8 with phosphoric acid）, the detective wavelength was 214nm. Results： Good linearlty was obtained in the range of 1.18 - 11.8ug （ r= 0.9999） .The average recovery was 98.52% with RSD of 0.85% （n = 6） .Conclusion： This method is simple, feasible and reproduciible. It can be used for the quality control of ZKT nanaoparticles.