期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Snapin蛋白与心肌L型钙离子通道Cav1.3的相互作用 被引量：1

Interaction between Snapin and Cardiac L-type Calcium Channel Cav1.3
下载PDF
导出
摘要 [目的]研究Snapin蛋白与心肌L型钙离子通道Cav1.3的相互作用。[方法]利用免疫共沉淀实验确定Snapin蛋白与Cav1.3在体外表达系统和心房组织存在相互作用。[结果]在过表达Snapin和Cav1.3的HEK293细胞及内源性表达二者的心房肌组织中,Snapin蛋白与Cav1.3钙离子通道存在相互作用。 [Objective] The aim was to study the interaction between Snapin and cardiac L-type calcium channel Cavl.3. [Method] Co-im- munoprecipitation assay was used to detect the physical interaction between Snapin and Carl. 3 in vitro expressing system and atrial tissues. [ Result] The results showed that interaction between Snapin and Carl. 3 existed either in the HEK293 cells heterologously co-expressed Snap- in with Cavl. 3 calcium channels or in mouse atrial tissues.
作者 袁秀杰 钱辉 刘金旭 张沙沙 石洁 赵钟钟 张朝
机构地区 南京师范大学生命科学学院江苏省分子医学生物技术重点实验室
出处 《安徽农业科学》 CAS 北大核心 2009年第26期12428-12429,共2页 Journal of Anhui Agricultural Sciences
基金 国家自然科学基金(30570662) 国家973重大科学研究计划(2006CB943503)
关键词 Snapin蛋白 Cav1.3钙离子通道 HEK293细胞 心房 Car1. 3 calcium channel HEK293 cells Atria
分类号 Q51 [生物学—生物化学]
  • 相关文献

参考文献11

  • 1TAKIMOTO K,LI D,NERBONNE J M,et al. Distribution,splicing and gluoocorticoid-indueed expression of cardiac alpha 1C and alpha ID volt- age-gated Ca^2+ channel mRNAs [ J ]. Journal of Molecular and Cellular Cardiology, 1997,29 ( 11 ) :3035 - 3042.
  • 2MANGONI M E,COUETTE B,BOURINET E,et al. Functional role of L- type Carl.3 Ca^2+ channels in cardiac pacemaker activity[J]. Proceedinss of the National Academy of Sciences of the United States of America,2003, 100(9) :5543 -5548.
  • 3XU W,LIPSCOMBE D. Neuronal Ca(V) 1.3alpha( 1 ) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines [ J ]. J Neurosci, 2001,21 ( 16 ) : 5944 - 5951.
  • 4ZHANG Z, XU Y, SONG H, et al. Functional Roles of Ca (v) 1.3 ( alpha (1D)) calcium channel in sinoatrial nodes: insight gained using gene-targeted null mutant mice[J]. Circulation Research,2002,90(9) :981 -987.
  • 5BELL D C,BUTCHER A J,BERROW N S,et al. Biophysical properties, pharmacology,and modulation of human, neuronal L-type ( alpha ( 1 D), Ca (V) 1.3) voltage-dependent calcium currents [ J ]. Journal of Neurophysiology,2001,85(2) :816 -827.
  • 6NAMKUNG Y,SKRYPNYK N,JEONG M J,et al. Requirement for the L- type Ca(2+ ) channel alpha(1D) subunit in postnatal pancreatic beta cell generation[J]. The Journal of Clinical Investigation,2001,108(7) :1015 - 1022.
  • 7BUXTON P,ZHANG X M,WALSH B,et al. Identification and characterization of Snapin as a ubiquitously expressed SNARE-binding protein that interacts with SNAP23 in non-neuronal cells[J]. The Biochemical Journal, 2003,375:433 - 440.
  • 8PAN P Y,CAI Q,LIN L,et al. SNAP-29-mediated modulation of synaptic transmission in cultured hippocampal neurons[J]. The Journal of Biological Chemistry,2005,280(27) :25769 -25779.
  • 9RUDER C,REIMER T, DELGADO-MARTINEZ I,et al. EBAG9 adds a new layer of control on large dense-core vesicle execytesis via interaction with Snapin [ J ]. Molecular Biology of the Cell ,2005,16(3) : 1245 - 1257.
  • 10ZISSIMOPOULOS S,WEST D J,WILUAMS A J,et al. Ryanodine receptor interaction with the SNARE-associated protein snapin [ J ]. Journal of Cell Science,2006,119:2386-2397.

同被引文献13

  • 1刘建军,黄海燕,庄志雄,李习艺,袁建辉,阳帆,卫秦芝.利用蛋白质组学技术研究三氯乙烯刺激肝细胞后蛋白质差异表达[J].中华劳动卫生职业病杂志,2005,23(6):431-434. 被引量：10
  • 2MADEIRA A,POMMET JM,PROCHIANTZ A,et al.SET protein(TAF1beta,I2PP2A) is involved in neuronal apoptosis induced by an amyloid precursor protein cytoplasmic subdomain[J].FASEB J,2005,19(13):1905-1907.
  • 3FUJIKI H,SUGANUMA M.Carcinogenic aspects of protein phosphatase 1 and 2A inhibitors[J].Prog Mol Subcell Biol,2009,46:221-254.
  • 4ANAZAWA Y,NAKAGAWA H,FURIHARA M,et al.PCOTH,a novel gene over expressed in prostate cancers,promotes prostate cancer cell growth through phosphorylation of oncoprotein TAF-Ibeta/SET[J].Cancer Res,2005,65(11):4578-4586.
  • 5TROTTA R,CIARLARIELLO D,DAL COL J,et al.The PP2A inhibitor SET regulates natural killer cell IFN-gamma production[J].J Exp Med,2007,204(10):2397-2405.
  • 6KARETSOU Z,EMMANOUILIDOU A,SANIDAS I,et al.Identification of distinct SET /T AF-I β domains required for core histone binding and quantitative characterisation of the interaction[J].BMC Biochemistry,2009,9(10):10-21.
  • 7SAITO S,MIYAJI-YAMAGUCHI M,NAGATA K.Aberrant intracellular localization of SET-CAN fusion protein,associated with a leukemia,disorganizes nuclear export[J].Int J Cancer,2004,111(4):501-507.
  • 8LI R,WANG H,BEKELE BN,et al.Identification of putative oncogenes in lung adenocarcinoma by a comprehensive functional genomic approach[J].Oncogene,2006,25(18):2628-2635.
  • 9TATSUKA M,MITSUI H,WADA M,et al.Elongation factor-1 alpha gene determines susceptibility to transformation[J].Nature,1992,359:333-336.
  • 10KIDO T,LAU YF.The human Y-encoded testis-specific protein interacts functionally with eukaryotic translation elongation factor eEF1A,a putative oncoprotein[J].Int J Cancer,2008,123(7):1573-1585.

引证文献1

二级引证文献2

安徽农业科学
2009年 第26期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部