不同预处理方法对饲料中微量元素含量测定的影响

Effects of Different Pretreatment Methods on Determination of Microelements in Feed

[目的]研究不同预处理方法对饲料中各微量元素含量测定结果的影响,找出各元素测定的最佳预处理方法。[方法]采用干灰化法、硝酸-高氯酸湿消化法和微波消解法对样品(精料和草料)进行预处理,利用电感耦合等离子原子发射光谱仪分别测定样品中A l、Ca、Cu、Fe、Mn、Se和Zn等7种微量元素含量。[结果]3种预处理方法都适合对精料中Cu、Mn、Zn和草料中Cu、Ca含量的测定,但利用硝酸-高氯酸湿消化法处理后,测定的草料中Cu和Ca含量较高;微波消解法适合精料中A l、Ca和草料中A l、Fe、Mn、Zn含量的测定;硝酸-高氯酸消化法适合精料中Fe含量的测定。[结论]测定饲料中不同微量元素的含量应选择不同的预处理方法。

[ Objective] To study the effects of different pretreatment methods on determination of microelement in feed, and thus find the best pretreatment method for determination of each microelement in feed. [ Method] The samples （concentrated feeding stuff and fodder） were pre- treated through three methods, that is, dry incineration method, HNO3 -HC104 wet-decomposition method and microwave digestion method. Then the content of sevcn kinds of microelement （Al, Ca, Cu, Fe, Mn, Se and Zn） was determined by inductively coupled plasma atomic emission spectrometer （ICP-AES）. [ Result] These three methods were all suitable for the determination of Cu, Mn and Zn in concentrated feeding stuff and the determination of Cu and Ca in fodder. The content of Cu and Ca was higher in fodder detected by HNO3 - HC104 wet-de- composition method. The microwave digestion method was suitable for the determination of Al and Ca in concentrated feeding stuff and the de- termination of Al, Fe, Mn and Zn in fodder. The dry incineration method was fit for the determination of Fe in concentrated feeding stuff. [ Conclusion ] The content of different microelements should be determined after the feed is treated with different pretreatment methods.